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huf  (ATCC)


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    ATCC huf
    Huf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/huf/product/ATCC
    Average 99 stars, based on 61 article reviews
    huf - by Bioz Stars, 2026-03
    99/100 stars

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    Metformin induces ZFP36 expression in cervical cancer-derived-cell lines. HeLa and CaSki cells were treated with metformin 20mM for 24 hours, then RNA was extracted for analysis by RT-qPCR. RT-qPCR of ZFP36 after metformin treatment in ( A ) HeLa and ( B ) CaSki. Representative Western Blot of ZFP36 and densitometric analysis in ( C ) HeLa and ( D ) CaSki, ( E ) HaCaT and ( F ) Human Uterine <t>Fibroblast</t> <t>(HUF).</t> Results are shown as means ± SD of at least three independent experiments. * p <0.05 **, p <0.01
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    primary human uterine fibroblast normal cells huf  (ATCC)
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    Effects of Pituranthos chloranthus (PC) and Teucrium ramosissimum Desf. (TR) extracts on normal primary human uterine <t>fibroblast</t> cells <t>(HUF)</t> and primary murine Bone Marrow-Derived Macrophages (BMDM) viability. After treatment of primary HUF and murine BMDM with increasing concentrations (0–100 µg/mL) of PC and TR for 72 h, the percentage of viable cells was assessed using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ( A ) Dose–response curves of PC-treated HUF (left panel) and TR-treated HUF (right panel). ( B ) Dose–response curves of PC-treated BMDM (left panel) and TR-treated BMDM (right panel). Data are expressed as a mean percentage of control growth ± Standard Deviation (SD) of two representative experiments ( n = 6 replicates per concentration).
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    Metformin induces ZFP36 expression in cervical cancer-derived-cell lines. HeLa and CaSki cells were treated with metformin 20mM for 24 hours, then RNA was extracted for analysis by RT-qPCR. RT-qPCR of ZFP36 after metformin treatment in ( A ) HeLa and ( B ) CaSki. Representative Western Blot of ZFP36 and densitometric analysis in ( C ) HeLa and ( D ) CaSki, ( E ) HaCaT and ( F ) Human Uterine Fibroblast (HUF). Results are shown as means ± SD of at least three independent experiments. * p <0.05 **, p <0.01

    Journal: BMC Cancer

    Article Title: Metformin induces ZFP36 by mTORC1 inhibition in cervical cancer-derived cell lines

    doi: 10.1186/s12885-024-12555-5

    Figure Lengend Snippet: Metformin induces ZFP36 expression in cervical cancer-derived-cell lines. HeLa and CaSki cells were treated with metformin 20mM for 24 hours, then RNA was extracted for analysis by RT-qPCR. RT-qPCR of ZFP36 after metformin treatment in ( A ) HeLa and ( B ) CaSki. Representative Western Blot of ZFP36 and densitometric analysis in ( C ) HeLa and ( D ) CaSki, ( E ) HaCaT and ( F ) Human Uterine Fibroblast (HUF). Results are shown as means ± SD of at least three independent experiments. * p <0.05 **, p <0.01

    Article Snippet: Primary Human Uterine Fibroblast (HUF) (PCS-460–010) and human cervical cancer-derived cell lines HeLa (RRID: CVCL_0030) and CaSki (RRID: CVCL_1100) were obtained from ATCC (Manassas, VA, USA).

    Techniques: Expressing, Derivative Assay, Quantitative RT-PCR, Western Blot

    Effects of Pituranthos chloranthus (PC) and Teucrium ramosissimum Desf. (TR) extracts on normal primary human uterine fibroblast cells (HUF) and primary murine Bone Marrow-Derived Macrophages (BMDM) viability. After treatment of primary HUF and murine BMDM with increasing concentrations (0–100 µg/mL) of PC and TR for 72 h, the percentage of viable cells was assessed using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ( A ) Dose–response curves of PC-treated HUF (left panel) and TR-treated HUF (right panel). ( B ) Dose–response curves of PC-treated BMDM (left panel) and TR-treated BMDM (right panel). Data are expressed as a mean percentage of control growth ± Standard Deviation (SD) of two representative experiments ( n = 6 replicates per concentration).

    Journal: Nutrients

    Article Title: Essential Oils, Pituranthos chloranthus and Teucrium ramosissimum , Chemosensitize Resistant Human Uterine Sarcoma MES-SA/Dx5 Cells to Doxorubicin by Inducing Apoptosis and Targeting P-Glycoprotein

    doi: 10.3390/nu13051719

    Figure Lengend Snippet: Effects of Pituranthos chloranthus (PC) and Teucrium ramosissimum Desf. (TR) extracts on normal primary human uterine fibroblast cells (HUF) and primary murine Bone Marrow-Derived Macrophages (BMDM) viability. After treatment of primary HUF and murine BMDM with increasing concentrations (0–100 µg/mL) of PC and TR for 72 h, the percentage of viable cells was assessed using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ( A ) Dose–response curves of PC-treated HUF (left panel) and TR-treated HUF (right panel). ( B ) Dose–response curves of PC-treated BMDM (left panel) and TR-treated BMDM (right panel). Data are expressed as a mean percentage of control growth ± Standard Deviation (SD) of two representative experiments ( n = 6 replicates per concentration).

    Article Snippet: Primary human uterine fibroblast normal cells (HUF) were obtained from the ATCC.

    Techniques: Derivative Assay, MTT Assay, Control, Standard Deviation, Concentration Assay